Anti-p40 - DeltaNp63 antibody [BC28] (ab172731)

Overview

  • Product name
    Anti-p40 - DeltaNp63 antibody [BC28]
    See all p40 - DeltaNp63 primary antibodies
  • Description
    Mouse monoclonal [BC28] to p40 - DeltaNp63
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human p40 - DeltaNp63 aa 5-17.
    Sequence:

    ENNAQTQFSEPQY


    Database link: Q9H3D4

  • Positive control
    • IHC-P: Human lung squamous cell carcinoma.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 20/07/17. The following lots are from ascites and are still in stock as of [20/07/17] – [GR311007, GR322490]. Lot numbers higher than [GR322490] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab172731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Relevance
    Differentiating between lung adenocarcinoma and squamous cell carcinoma is an important distinction with therapeutic implications. Although the most frequently recommended squamous marker p63 is extremely sensitive, it suffers from low specificity due to its reactivity in a substantial proportion of lung adenocarcinomas and other tumor types, particularly lymphomas. p40 is a relatively unknown marker that recognizes ?Np63-a p63 isoform suggested to be highly specific for squamous/basal cells. p40 is equivalent to p63 in sensitivity for squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma.
  • Database links

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lung squamous cell carcinoma labelling p40 - DeltaNp63 with ab172731 for 30 minutes at room temperature. Counterstained with hematoxylin.

References

ab172731 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Adenosquamous tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Adenosquamous tumor
Blocking step
Dako Protein Block, serum-free as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
Formaldehyde
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Verified customer

Submitted Jun 18 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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