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Synthetic peptide derived from residues 200 - 300 of Human p400.
Our Abpromise guarantee covers the use of ab5201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 19000314|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/1000. Predicted molecular weight: 344 kDa.|
ICC/IF image of ab5201 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 10 min) and incubated with the antibody (ab5201, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Rabbit polyclonal to p400 used at 1/1000. p400 is part of the Tip60/NuA4 HAT complex.
Nuclear extracts from Mock (1), Tip60 (2) and an unrelated TAP-tagged protein (3) were purified by tandem affinity purification. No non-specific bands are observed.
Whilst it is our normal policy to display the full gel for Western Blots, in this case we received a strip image from an academic collaborator. However, we still feel this image provides useful information on ab5201.Image from PMID:16387653 Western blot analysis of p400 antibody using TAP purifications of ING4 and Tip60 that shows two distinct complexes