Anti-p400 antibody (ab70301)
Key features and details
- Rabbit polyclonal to p400
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p400 antibody
See all p400 primary antibodies -
Description
Rabbit polyclonal to p400 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between C terminal residues 3075 and 3124 of human p400
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70301 was affinity purified using an epitope specific to p400 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/5000. Detects a band of approximately 470 kDa (predicted molecular weight: 344 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/5000. Detects a band of approximately 470 kDa (predicted molecular weight: 344 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 Myb-like domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57634 Human
- Omim: 606265 Human
- SwissProt: Q96L91 Human
- Unigene: 595201 Human
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Alternative names
- CAG repeat protein 32 antibody
- CAGH 32 antibody
- CAGH32 antibody
see all
Images
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Anti-p400 antibody (ab70301) at 0.4 µg/ml + NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 344 kDa
Exposure time: 30 seconds -
All lanes : Anti-p400 antibody (ab70301) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K-562 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 344 kDa
Exposure time: 30 seconds -
Immunoprecipitation of ab70301 using HeLa cells.
Lane 1: rabbit anti-p400 antibody
Lane 2: ab70301 current lot
Lane 3: ab70301 previous lot
Lane4: Control IgG
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab70301 has been referenced in 8 publications.
- Moreno-Andrés D et al. VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis. Cells 9:N/A (2020). PubMed: 32708675
- Moen MJ et al. An interaction network of mental disorder proteins in neural stem cells. Transl Psychiatry 7:e1082 (2017). WB . PubMed: 28375211
- Pradhan SK et al. EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation. Mol Cell 61:27-38 (2016). PubMed: 26669263
- Pérez-García V et al. Cell activation-induced phosphoinositide 3-kinase alpha/beta dimerization regulates PTEN activity. Mol Cell Biol 34:3359-73 (2014). WB ; Mouse . PubMed: 24958106
- Bellucci L et al. Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z. PLoS One 8:e54102 (2013). ChIP ; Human . PubMed: 23349794
- Mattera L et al. The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis. PLoS Genet 6: (2010). WB ; Human . PubMed: 20548951
- Kim J et al. A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs. Cell 143:313-24 (2010). WB ; Mouse . PubMed: 20946988
- Young AP et al. VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400. Nat Cell Biol 10:361-9 (2008). PubMed: 18297059