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Western blot - p48 CAF1 antibody (ab1765)
(enlarge)
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Western blot - p48 CAF1 antibody (ab1765)
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Immunoprecipitation - p48 CAF1 antibody (ab1765)
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Product Name
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RbAp48 antibody
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See all RbAp48 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RbAp48
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Immunogen
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Synthetic peptide: CENIYNDEDPEGSVDPEGQGS, corresponding to amino acids 406-425 of Human and Mouse CAF 1 (p48).
BLAST 'CENIYNDEDPEGSVDPEGQGS' with  or 
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Chk, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: use at a dilution of 1/50.
IP: Use at a concentration of 20 µg/ml.
WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Rb
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> ISWI/NURF/CHRAC
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Relevance
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RbAp48 (Retinoblastoma-binding protein p48 or Rb-associated protein p48)is a WD repeat protein that is a core histone binding subunit common to several complexes involved in chromatin assembly, chromatin remodeling, and histone deacetylation. These complexes include Chromatin Assembly Factor 1 (CAF1), which is required for chromatin assembly following DNA replication and repair, the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and subsequent transcriptional repression, the nucleosome remodeling and histone deacetylation complex NuRD, the nucleosome remodeling factor (NURF) complex, and the PRC2 complex, which promotes repression of homeotic genes during development. RpAp48 also interacts with the retinoblastoma protein, and with SPEN/MINT and BRCA1. It is also a component of the DREAM complex, which represses cell cycle-dependent genes in quiescent cells.
A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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serum with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Locus link: 350005
Alternative names: similar to chromatin assembly factor 1 p48 subunit
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RbAp48 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RbAp48 antibody images, references, products related to ab1765 and other tools.
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RbAp48 antibody images:
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 Western blot - p48 CAF1 antibody (ab1765)
Predicted band size : 47.6 kDa
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Western blot - p48 CAF1 antibody (ab1765)
RbAp48 antibody (ab1765) at 1/1000 dilution
Secondary
Anti-rabbit IgG HRP
developed using the ECL technique
Predicted band size : 47.6 kDa
Observed band size : 40,55 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
ab1765 detecting p48 CAF1 from human LCL whole cell lysates by Western Blot.
See Abreview
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Immunoprecipitation - p48 CAF1 antibody (ab1765)
ab1765 used in Immunoprecipitation of HeLa whole cell lysate. Whole cell extract (containing 100µg of soluble protein) was incubated with 20µg of antibody overnight. Protein A-Agarose was added to the immunocomplexes and incubated at room temperature for three hours. Immunocomplexes were eluted and then resolved using 7.5% SDS-PAGE under reducing conditions. For the detection of HDAC1, 2 and 3 a non-Abcam mouse monoclonal antibody was used at a dilution of 1/800. Results support the understanding that HDAC1 and 2 are in the same complex together with p48. Note that HDAC1 does not coprecipitate HDAC2, probably displaced by the antibody during the experiment. Lane 1: Input Lane 2: HDAC1 (ab1769) Lane 3: p48 (ab1765) Lane 4: No antibody control This image is courtesy of an Abreview by Hugh Spotswood submitted on 9 February 2006.
See Abreview
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Immunocytochemistry/ Immunofluorescence - p48 CAF1 antibody (ab1765)
ab1765 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-rabbit antibody was used as the secondary. This image is courtesy of an Abreview submitted by Dr Alexander Rapp
See Abreview
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References for RbAp48 antibody (ab1765)
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This product has been used in: (two most recent references)
Pegoraro G et al. Ageing-related chromatin defects through loss of the NURD complex. Nat Cell Biol 11:1261-7 (2009). ICC/IF; Human. PubMed: 19734887
Nitzsche A et al. Temporal Dynamics of Cytomegalovirus Chromatin Assembly in Productively Infected Human Cells. J Virol : (2008). WB, ICC/IF; Human. PubMed: 18786996
If you publish research using ab1765 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RbAp48
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RbAp48 antibody - more information
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Customer reviews (feedback) regarding RbAp48 antibody |
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RbAp48 antibody - related products:
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RbAp48 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RbAp48 antibody |
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