Overview

  • Product nameAnti-P4HA1 antibody
    See all P4HA1 primary antibodies
  • Description
    Goat polyclonal to P4HA1
  • SpecificityThis antibody is expected to recognise both reported isoforms.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Dog, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide:

    C-DFARKDEPDAFKE

    , corresponding to internal sequence amino acids 431-443 of Human P4HA1

  • Positive control
    • HEK293 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide.

Target

Anti-P4HA1 antibody images

  • Anti-P4HA1 antibody (ab59497) at 0.2 µg/ml + HEK293 cell lysate (35µg protein in RIPA buffer)

    Predicted band size : 61 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC image of ab59497 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59497, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-P4HA1 antibody (ab59497) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from 9L cells
    Lane 2 : Whole cell lysate prepared from PC12 cells
    Lane 3 : Whole cell lysate prepared from RIE-1 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-P4HA1 antibody (ab59497)

This product has been referenced in:
  • Wang XL  et al. Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-?B Pathway. PLoS One 11:e0153919 (2016). WB ; Human . Read more (PubMed: 27120457) »
  • Wong L  et al. Prostatic inflammation induces fibrosis in a mouse model of chronic bacterial infection. PLoS One 9:e100770 (2014). IHC-P ; Mouse . Read more (PubMed: 24950301) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human cancer cell lines)
Loading amount 20 µg
Specification Human cancer cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Rat cell lines)
Loading amount 20 µg
Specification Rat cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF, F9, GC-1, NIH-3T3)
Loading amount 20 µg
Specification MEF, F9, GC-1, NIH-3T3
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"