Overview

  • Product nameAnti-P4HA2 antibody
    See all P4HA2 primary antibodies
  • Description
    Rabbit polyclonal to P4HA2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 250 and 300 of human P4HA2. (NP_004190.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionCatalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
  • Sequence similaritiesBelongs to the P4HA family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 TPR repeat.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
    • 4 PH alpha 2 antibody
    • 4-PH alpha-2 antibody
    • C-P4Halpha(II) antibody
    • collagen prolyl 4-hydroxylase alpha(II) antibody
    • P4HA2 antibody
    • P4HA2_HUMAN antibody
    • procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II antibody
    • procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2 antibody
    • Procollagen-proline antibody
    • procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody
    • Prolyl 4 hydroxylase alpha 2 subunit antibody
    • Prolyl 4 hydroxylase subunit alpha 2 [Precursor] antibody
    • Prolyl 4-hydroxylase subunit alpha-2 antibody
    • Prolyl 4-hydroxylase, alpha polypeptide II antibody
    see all

Anti-P4HA2 antibody images

  • All lanes : Anti-P4HA2 antibody (ab70887) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 28 kDa,33 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70887 at 1 µg/ml for WB subsequent to IP at 3 µg/mg lysate.
    Lane 2: control IgG.
    Detection: Chemiluminescence with an exposure time of 30 seconds.
  • All lanes : Anti-P4HA2 antibody (ab70887) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from 9L cells
    Lane 2 : Whole cell lysate prepared from PC12 cells
    Lane 3 : Whole cell lysate prepared from RIE-1 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 35 kDa (possible degradation product).

    Exposure time : 5 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-P4HA2 antibody (ab70887)

ab70887 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Rat cell lines)
Loading amount 20 µg
Specification Rat cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Submitted Feb 22 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"