Overview

  • Product name
  • Description
    Rabbit polyclonal to P4HA3
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 130-396 of Human P4HA3 (BC117333).

  • Positive control
    • Human fetal kidney & liver lysates, Human fetal liver tissue.

Properties

  • Form
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
  • Tissue specificity
    Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.
  • Sequence similarities
    Belongs to the P4HA family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 TPR repeat.
  • Post-translational
    modifications
    N-glycosylation plays no role in the catalytic activity.
  • Cellular localization
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-oxoglutarate-4-dioxygenase subunit alpha-3 antibody
    • 4-PH alpha-3 antibody
    • P4ha3 antibody
    • P4HA3_HUMAN antibody
    • Procollagen-proline antibody
    • Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III antibody
    • Procollagen-proline, 2-oxoglutarate 4-dioxygenase, alpha subunit, isoform 3 antibody
    • Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3 antibody
    • Prolyl 4-hydroxylase subunit alpha-3 antibody
    • Prolyl 4-hydroxylase, alpha polypeptide III antibody
    • Prolyl 4-hydroxylase, alpha-3 subunit antibody
    see all

Anti-P4HA3 antibody images

  • All lanes : Anti-P4HA3 antibody (ab101657) at 1/500 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate


    Predicted band size : 61 kDa
  • ab101657 at 1/100 dilution staining P4HA3 in Human fetal liver by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

References for Anti-P4HA3 antibody (ab101657)

This product has been referenced in:
  • Olivares O  et al. Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions. Nat Commun 8:16031 (2017). WB, IHC . Read more (PubMed: 28685754) »
  • Akamatsu T  et al. Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences. Fibrogenesis Tissue Repair 6:15 (2013). IHC-P . Read more (PubMed: 23927729) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab101657.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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