Overview

  • Product nameAnti-P4HA3 antibody
    See all P4HA3 primary antibodies
  • Description
    Rabbit polyclonal to P4HA3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 130-396 of Human P4HA3 (BC117333).

  • Positive control
    • Human fetal kidney & liver lysates, Human fetal liver tissue.

Properties

  • FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/500.

Target

  • FunctionCatalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
  • Tissue specificityHighly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions.
  • Sequence similaritiesBelongs to the P4HA family.
    Contains 1 Fe2OG dioxygenase domain.
    Contains 1 TPR repeat.
  • Post-translational
    modifications
    N-glycosylation plays no role in the catalytic activity.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-oxoglutarate-4-dioxygenase subunit alpha-3 antibody
    • 4-PH alpha-3 antibody
    • P4ha3 antibody
    • P4HA3_HUMAN antibody
    • Procollagen-proline antibody
    • Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III antibody
    • Procollagen-proline, 2-oxoglutarate 4-dioxygenase, alpha subunit, isoform 3 antibody
    • Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3 antibody
    • Prolyl 4-hydroxylase subunit alpha-3 antibody
    • Prolyl 4-hydroxylase, alpha polypeptide III antibody
    • Prolyl 4-hydroxylase, alpha-3 subunit antibody
    see all

Anti-P4HA3 antibody images

  • All lanes : Anti-P4HA3 antibody (ab101657) at 1/500 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate


    Predicted band size : 61 kDa
  • ab101657 at 1/100 dilution staining P4HA3 in Human fetal liver by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

References for Anti-P4HA3 antibody (ab101657)

This product has been referenced in:
  • Akamatsu T  et al. Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences. Fibrogenesis Tissue Repair 6:15 (2013). IHC-P . Read more (PubMed: 23927729) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"