Overview

  • Product nameAnti-P4HB antibody
    See all P4HB primary antibodies
  • Description
    Rabbit polyclonal to P4HB
  • SpecificityRecognizes endogenous levels of total P4HB.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH, derived from the sequence around Proline 329 of Human P4HB

  • Positive control
    • Human formalin fixed paraffin embedded samples: Pancreatic exocrine cells, Lung macrophages, type 2 pneumocytes, Hepatocytes, Renal tubules, Placenta trophoblasts. Small intestine tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. We can recommend to use pH 6.0 citrate buffer.
ICC/IF 1/50.

Methanol permeabilization is recommended.

Target

  • FunctionThis multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
  • Sequence similaritiesBelongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localizationEndoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular thyroid hormone binding protein antibody
    • Cellular thyroid hormone-binding protein antibody
    • Collagen prolyl 4 hydroxylase beta antibody
    • Disulphide Isomerase antibody
    • DSI antibody
    • EC 5.3.4.1 antibody
    • Endoplasmic reticulum resident protein 59 antibody
    • ER protein 59 antibody
    • ERBA2L antibody
    • ERp59 antibody
    • GIT antibody
    • Gltathione insulin transhydrogenase antibody
    • Glutathione insulin transhydrogenase antibody
    • P4HB antibody
    • P4Hbeta antibody
    • p55 antibody
    • PDI antibody
    • PDIA1 antibody
    • PDIA1_HUMAN antibody
    • PDIR antibody
    • PHDB antibody
    • PO4DB antibody
    • PO4HB antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit antibody
    • PROHB antibody
    • Prolyl 4 hydroxylase beta polypeptide antibody
    • Prolyl 4 hydroxylase beta subunit antibody
    • Prolyl 4 hydroxylase subunit beta antibody
    • Prolyl 4-hydroxylase subunit beta antibody
    • Protein disulfide isomerase associated 1 antibody
    • Protein disulfide isomerase, family A, member 1 antibody
    • Protein disulfide isomerase/oxidoreductase antibody
    • Protein disulfide-isomerase antibody
    • Protocollagen hydroxylase antibody
    • Thbp antibody
    • Thyroid hormone binding protein p55 antibody
    • Thyroid hormone binding protein p55 cellular antibody
    • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like antibody
    see all

Anti-P4HB antibody images

  • ab85564 at a 1/50 dilution staining P4HB in human small intestine tissue by IHC-P.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue, staining P4HB with ab85564 at 1/50 dilution.

References for Anti-P4HB antibody (ab85564)

This product has been referenced in:
  • Koga K  et al. Macrophage migration inhibitory factor provides cardioprotection during ischemia/reperfusion by reducing oxidative stress. Antioxid Redox Signal 14:1191-202 (2011). WB ; Mouse . Read more (PubMed: 20831446) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"