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p53 (TP53 gene) acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. p53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. p53 mediated apoptosis induction seems to be by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. p53 is also implicated in Notch signaling cross-over.
ab124533 p53 Phospho S392 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the accurate quantitative measurement of phosphorylated Ser392 of p53 protein in human cell and tissue lysates. The assay employs a p53 protein specific antibody coated onto well plate strips.
Samples are pipetted into the wells and p53 present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-p53 phospho Ser392 detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phosphorylated Ser392 of bound p53. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
ab124533 detects phosphorylated Ser392 of p53 in Human samples only.
Typical working ranges:
Hek293T cells (Etoposide treated): 12 – 800 µg/ml
MCF7 cells (Camptothecin treated): 12 – 400 µg/ml
Store all components at 4°C. This kit is stable for 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed. After opening, the unused Incubation Buffer should be stored at -20°C.
|Components||1 x 96 tests|
|10X HRP Label||1 x 1ml|
|10X p53 Phospho S392 Detector Antibody||1 x 0.7ml|
|10X Wash Buffer||1 x 40ml|
|Extraction Buffer||1 x 15ml|
|Incubation Buffer||1 x 60ml|
|p53-coated Microplate||1 unit|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab124533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab124533 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"