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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p53 (acetyl K386) antibody (ab52172)
ab52172 at 1/50 dilution staining P53 (acetyl K386) in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence (right image) of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - p53 (acetyl K386) antibody (ab52172)
ICC/IF image of ab52172 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52172, 1µg/ml) overnight at +4°C. The secondary antibody (green)was Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43$%micro;M.
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