p53 (acetyl K386) antibody (ab52172)

Product Name 

p53 (acetyl K386) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to p53 (acetyl K386)

Immunogen 

Synthetic acetylated peptide derived from human p53 around the acetylation site of lysine 386 (M-F-K^A-T-E).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab52172 detects endogenous levels of p53 only when acetylated at
lysine 386.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/40000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/50 - 1/100.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human lung carcinoma tissue.

Cellular localization 

Cytoplasmic, Endoplasmic reticulum and Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53

Relevance 

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. p53 is phosphorylated at Ser6 and Ser9 by ck1d and ck1e both in vitro and in vivo. Phosphorylation of p53 at Ser46 is important in regulating the ability of p53 to induce apoptosis. In vivo phosphorylation of p53 at Ser33 by cdk7/cyclin H and in response to UV irradiation has been observed.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab52172 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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