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Recombinant full length protein (wild type) (Human).
Our Abpromise guarantee covers the use of ab7757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200. 1/200 - please refer to abreview for further protocol details.|
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
p53 was immunoprecipitated using 0.5mg T47D whole cell extract, 5µg of Rabbit polyclonal to Mouse monoclonal to p53 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, T47D whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7757.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 53kDa; p53
Image courtesy of an Abreview submitted by Santhosh Gopal.
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