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ab28 has been referenced in 6 publications.
Publishing research using ab28? Please let us know so that we can cite the reference in this datasheet
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Ab28 staining human normal kidney medulla. Staining is localized to cytoplasm and endoplasmic reticulum, and faint nuclear staining is also present.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab28 staining p53 in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4ºC. The secondary antibody used was a goat anti-mouse IgG conjugated to Cy3® used at a 1/400 dilution.
Image courtesy of an anonymous Abreview.
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