Anti-p53 antibody [PAb 1801] (ab28)

The concentration for human tissue we tried 1:500, 1:800and 1:1000.

For rat liver tissue we tried 1:1000.

Please refund us back.

Thanks

ANSWER:

Thank you for contacting us.

Your credit note ID is **********.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

At the moment I am trying to investigate the p53 activity in my cell line using the western blot technique. I am using a antibody for total p53 and a phospho-p53 (Ser15) antibody. I ordered those antibodies from a different company. However I am not completely happy with those antibodies. Therefore I am looking into ordering new antibodies.

I was wondering which are the conditions you optimised your p53 antibody (ab26 or ab28) and phospho-p53 (Ser15) antibody (amount of protein needed, Blotiing solution and time, etc.)? How long would the shipping be taking?

ANSWER:

Thank you for contacting us.

Our p53 antibody ab26 has been optimized by loading 20 ug per lane of HeLa cells, using 5% BSA as the blocking agent for 1 hour at room temperature, and incubating with the primary antibody at a concentration of 5ug / mL overnight at 4C. To view the protocols used by our customers, you can browse our WB Abreviews for this product:

http://www.abcam.com/p53-antibody-PAb-240-ab26-reviews.html#WB

Ab28 and ab1431 can be used with a similar protocol, loading 20-40 ug mer lane, using a 5% milk block, and a primary antibody dilution of 1:1000.

We will ship anywhere in the UK or US overnight for next day delivery. I hope this helps, please let me know if you need any additional information or assistance.

Hello,

We have an item complaint. Please check attachment.

ANSWER:

Thank you for contacting Abcam.

I am sorry about all the problems you have been having with ab28 in western blot. I just had one more protocol that you used.:

What primary antibody concnetrations did you try?



I look forward to your rpely and helping you resolve this issue. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on rat and humansamples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

I want to do a rat liver immunohistochemical staining of BCL2, p53, Ki67, Bax. Having no experience with it, I would ask you to tell me about the price, the primary and secondary antibodies, as well as all other reagents that I need to do this staining wich are compatibile with rat tissue and with each other. Thank you

ANSWER:

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

We have many BCl2 products in catalogue however I can recomend using ab18210 as it was tested in Immunohistochemistry on rat tissue sections.

ab18210: http://www.abcam.com/Bcl2-antibody-ab18210.html

The recomended p53 antibodies would be

ab26; http://www.abcam.com/p53-antibody-P-ab26.html ab28; http://www.abcam.com/p53-antibody-P-ab28.html ab2433; http://www.abcam.com/p53-antibody-ab2433.html

The recommended Ki67 antibodies are;

ab66155; http://www.abcam.com/Ki67-antibody-ab66155.html ab16667; http://www.abcam.com/Ki67-antibody-SP6-Proliferation-Marker-ab16667.html

We have region specific prices for the antibodies. The best way to know the price is by selecting the country of your origin displayed on top right corner of the datasheet. The system will automatically give you the price and shipping charges.

Please be also advised that you would also need fluorophore or enzyme conjugated secondary antibodies. I have attached a protocol booklet where protocol of each application has been nicely explained including the ingredients required. Please go through it and decide which antibody and reagent you require.

I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

Using this Ab, I get a distinct band at about 53kD and one at about 73KD with minor bands at less than 50Kd. Does this Ab recognize p73 or other proteins?

ANSWER:

It is very common to see multiple bands when using an antibody to p53. Your band at 73kDa is almost certainly p73 as this protein shows sequence homology to p53 and will be recognised by this antibody. Other bands may be due to the different modificaiton states of p53, although we'd typically expect these to run at over 53 kDa.