Western blot

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Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Cell lysate - whole cell (Mouse cardiomyocytes)
Specification Mouse cardiomyocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution 1/2000

Secondary antibody

Name Non-Abcam antibody was used: Anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/3000


Detection method ECL
Bands Specific: Around 55 kDa
Exposure 30 second(s)

Additional data

Additional Notes Three different lysates from adult mouse cardiomyocytes

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Submitted May 21 2013