Western blot

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Cell lysate - whole cell (Mouse cardiomyocytes)
Specification
Mouse cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Bands
Specific: Around 55 kDa
Exposure
30 second(s)

Additional data

Additional Notes
Three different lysates from adult mouse cardiomyocytes
Username

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Verified customer

Submitted May 21 2013

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