Western blot abreview for Anti-p53 antibody [PAb 240]

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Mouse Tissue lysate - whole (Mouse Testis)
Specification Mouse Testis
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Concentration 5 µg/ml
Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween

Secondary antibody

Name Non-Abcam antibody was used: IRD 800 CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution 1/15000


Detection method Infrared Odyssey
Bands Specific: 53 kDa
Exposure 4 minute(s) and 0 second(s)

Additional data

Additional Notes 1= Marker 2= Mouse Testis

Dr. Francesco Elia Marino

Verified customer

Submitted May 15 2014