Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Tissue lysate - whole (Mouse Testis)
Specification
Mouse Testis
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Concentration
5 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween

Secondary antibody

Name
Non-Abcam antibody was used: IRD 800 CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution
1/15000

Detection

Detection method
Infrared Odyssey
Bands
Specific: 53 kDa
Exposure
4 minute(s) and 0 second(s)

Additional data

Additional Notes
1= Marker 2= Mouse Testis
Username

Dr. Francesco Elia Marino

Verified customer

Submitted May 15 2014

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