HCT116 p53-/- cells were used as negative control. Immunodetection was performed with a non-abcam anti p53 antibody and a fluorescence labeled secondary antibody from abcam. Dynabeads Protein G were cross linked with 6.5 ´g antibody per mg beads.
The antibody works specifically, but the amount of immunoprecipitated p53 could be higher.
Dr. Oliver Maddocks
Submitted Jun 22 2014
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