Immunoprecipitation abreview for Anti-p53 [PAb 240] antibody

Application Immunoprecipitation
Immuno-precipitation step Other - Dynabeads Protein G
Sample Human Cell lysate - whole cell (HCT116 human colon carcinoma cell line)
Specification HCT116 human colon carcinoma cell line
Treatment 15 ´M etoposide for 24 hrs
Total protein in input 7e+006 cells

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: lysis buffer
Dilution 1/150

Western Blot antibody

Dilution 1/5000

Additional data

Additional Notes HCT116 p53-/- cells were used as negative control. Immunodetection was performed with a non-abcam anti p53 antibody and a fluorescence labeled secondary antibody from abcam. Dynabeads Protein G were cross linked with 6.5 ´g antibody per mg beads. The antibody works specifically, but the amount of immunoprecipitated p53 could be higher.

Dr. Oliver Maddocks

Verified customer

Submitted Jun 22 2014