Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 antibody [PAb 240]

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde

Other product details

Incubation time 14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution 1/500

Secondary antibody

Name Non-Abcam antibody was used: Alexa Fluor­ 594 Goat Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution 1/500

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Submitted Mar 26 2014