Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 antibody [PAb 240]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (Human primary fibroblasts)
Specification
Human primary fibroblasts
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor­ 594 Goat Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
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Submitted Mar 26 2014

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