Western blot abreview for Anti-p53 antibody [PAb 240]

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (MDA-MB-231)
Specification MDA-MB-231
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Concentration 2 µg/ml
Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS

Secondary antibody

Name Non-Abcam antibody was used: Goat anti-mouse IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/2000


Detection method ECL select
Bands Specific: ~50 kDa Non-specific: 80, 150 kDa
Exposure 5 minute(s) and 0 second(s)
Positive control MDA-MB-231

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Submitted Aug 07 2014