Flow Cytometry abreview for Anti-p53 antibody [PAb 240]

Application Flow Cytometry
Fixation Methanol
Permeabilization Yes - 0.1% Tween
Sample Human Cell (MDA-MB-231)
Specification MDA-MB-231
Gating Strategy Live cells gated
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free

Other product details

Concentration 1 µg/cells
Incubation time 1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS + 0.2% BSA + 0.1% Sodium azide

Secondary antibody

Name Non-Abcam antibody was used: Chicken anti-mouse IgG PE
Host species: Chicken
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution 1/100

Additional data

Additional Notes The purple plot represents the isotype control and the green plot represents p53 in MDA-MB-231 cells.
Abcam response Thank you for sharing this data with us. ab26 has previously been tested in flow cytometry on HeLa cells (please see image on datasheet). However, based on this abreview, it is clear that this antibody does not work in flow on MDA-MB-231 cells, under these experimental conditions. Abcam would like to hear from other customers using this antibody for flow cytometry.

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Submitted Dec 29 2014