Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 antibody [PAb 240]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (MDA-MB-231)
Specification
MDA-MB-231
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde

Other product details

Concentration
5 µg/ml
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 0.1% BSA+1% goat serum+0.1%Triton in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse IgG1
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
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Submitted Sep 23 2014

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