Western blot abreview for Anti-p53 antibody [PAb 240]

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (LNCaP)
Specification LNCaP
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution 1/500

Secondary antibody

Name Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution 1/10000


Detection method Infrared
Bands Specific: 53 kDa
Exposure 4 minute(s) and 0 second(s)

Additional data

Additional Notes Picture shows LNCaP cells in replicates of 4.

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Submitted Jan 16 2015