Western blot abreview for Anti-p53 antibody [PAb 240]

Excellent
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (LNCaP)
Specification
LNCaP
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution
1/10000

Detection

Detection method
Infrared
Bands
Specific: 53 kDa
Exposure
4 minute(s) and 0 second(s)

Additional data

Additional Notes
Picture shows LNCaP cells in replicates of 4.
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Submitted Jan 16 2015

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