Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Application Western blot
Sample Dog Cell lysate - whole cell (CPEK)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification CPEK
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time 16 hour(s) and 0 minute(s)
Dilution 1/500

Secondary antibody

Dilution 1/10000
Name Non-Abcam antibody was used: anti-Mouse (H+L)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method ECL
Exposure 15 second(s)
Bands Specific: 53 kDa Non-specific: 90 kDa

Additional data

Additional Notes longer exposures resulted in some more non-specific bands. not sure if some of the bands could represent modified p53.
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Submitted Apr 17 2015