Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Application
Western blot
Sample
Dog Cell lysate - whole cell (CPEK)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
CPEK
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-Mouse (H+L)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 53 kDa Non-specific: 90 kDa

Additional data

Additional Notes
longer exposures resulted in some more non-specific bands. not sure if some of the bands could represent modified p53.
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Submitted Apr 17 2015

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