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Immunocytochemistry/ Immunofluorescence - p53 antibody (ab1101)
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Western blot - p53 antibody (ab1101)
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Product Name
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p53 antibody
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See all p53 antibodies (80)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to p53
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Immunogen
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Recombinant human p53 expressed in E. coli.
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Reacts with
(species key)
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Hu
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Epitope
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This antibody recognizes an epitope within aa 20-25 at the N-terminus of human p53.
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Tested applications
(see key)
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ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 1 - 5 µg/ml.
Flow Cyt: Use at a concentration of 1 - 5 µg/ml.
ICC/IF: Use at an assay dependent dilution. (Abreview: 6819)
IHC-P: Use at a concentration of 1 - 5 µg/ml.
IHC-Fr: Use at a concentration of 1 - 5 µg/ml.
IP: Use at a concentration of 1 - 5 µg/ml.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HBL 100 cells.
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Cellular localization
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Cytoplasmic, Endoplasmic reticulum and Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Relevance
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p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. p53 is phosphorylated at Ser6 and Ser9 by ck1d and ck1e both in vitro and in vivo. Phosphorylation of p53 at Ser46 is important in regulating the ability of p53 to induce apoptosis. In vivo phosphorylation of p53 at Ser33 by cdk7/cyclin H and in response to UV irradiation has been observed.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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PBS, pH7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this p53 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for p53 antibody images, references, products related to ab1101 and other tools.
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p53 antibody images:
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 Immunocytochemistry/ Immunofluorescence - p53 antibody (ab1101)
ICC/IF image of ab1101 stained human HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1101, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and MCF7 cells.
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Western blot - p53 antibody (ab1101)
All lanes : p53 antibody (ab1101) at 2.5 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 55 kDa (possible post-translational modification).
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References for p53 antibody (ab1101)
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This product has been used in: (two most recent references)
Ghavami S et al. Statin-triggered cell death in primary human lung mesenchymal cells involves p53-PUMA and release of Smac and Omi but not cytochrome c. Biochim Biophys Acta 1803:452-67 (2010). WB; Human. PubMed: 20045437
Ottewell PD et al. Anticancer mechanisms of doxorubicin and zoledronic acid in breast cancer tumor growth in bone. Mol Cancer Ther 8:2821-32 (2009). WB; Mouse. PubMed: 19789217
See all 3 publication references for this product.
If you publish research using ab1101 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to p53
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p53 antibody - more information
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Customer reviews (feedback) regarding p53 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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