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ab2434 |
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ab2433 has been referenced in 5 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab2433 shown recognizing mouse and human p53 in Western blot. It was used at a 1/200 dilution for western on A549, MCF-7 human cells and B16F10. This picture was kindly submitted by Meijuan Zhou as part of the review on this product.
ICC/IF image of ab2433 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2433, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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