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- p53 antibody (ab2433)
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Immunocytochemistry/ Immunofluorescence - p53 antibody (ab2433)
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Product Name
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p53 antibody
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See all p53 antibodies (79)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to p53
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Immunogen
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Synthetic peptide: CPGRDRRTEEENLRKKGEPH , corresponding to Internal sequence amino acids 277-296 of Human p53
(Peptide available as ab2434.)
BLAST 'CPGRDRRTEEENLRKKGEPH' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/200. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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A-431 lysate.
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Cellular localization
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Cytoplasmic, Endoplasmic reticulum and Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
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Relevance
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p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. p53 is phosphorylated at Ser6 and Ser9 by ck1d and ck1e both in vitro and in vivo. Phosphorylation of p53 at Ser46 is important in regulating the ability of p53 to induce apoptosis. In vivo phosphorylation of p53 at Ser33 by cdk7/cyclin H and in response to UV irradiation has been observed.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this p53 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for p53 antibody images, references, products related to ab2433 and other tools.
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p53 antibody images:
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 - p53 antibody (ab2433)
ab2433 shown recognizing mouse and human p53 in Western blot. It was used at a 1/200 dilution for western on A549, MCF-7 human cells and B16F10. This picture was kindly submitted by Meijuan Zhou as part of the review on this product.
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Immunocytochemistry/ Immunofluorescence - p53 antibody (ab2433)
ICC/IF image of ab2433 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2433, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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References for p53 antibody (ab2433)
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This product has been used in: (two most recent references)
Koues OI et al. Regulation of acetylation at the major histocompatibility complex class II proximal promoter by the 19S proteasomal ATPase Sug1. Mol Cell Biol 28:5837-50 (2008). WB; Human. PubMed: 18662994
Brady M et al. Regulation of p53 and MDM2 activity by MTBP. Mol Cell Biol 25:545-53 (2005). WB; Human. PubMed: 15632057
If you publish research using ab2433 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to p53
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p53 antibody - more information
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Customer reviews (feedback) regarding p53 antibody |
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Contact Abcam with a Technical Enquiry about p53 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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