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Synthetic peptide within Human p53 aa 277-296 (internal sequence). The exact sequence is proprietary.
Our Abpromise guarantee covers the use of ab2433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).Can be blocked with Human p53 peptide (ab2434).|
|ICC/IF||Use a concentration of 1 mg/ml.|
|Flow Cyt||Use at an assay dependent concentration.|
ICC/IF image of ab2433 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2433, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L)ab150077) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab2433 shown recognizing mouse and human p53 in Western blot. It was used at a 1/200 dilution for western on A549, MCF-7 human cells and B16F10. Predicted Band 53 kDa.
ab2433 staining p53 in human ovarian carcinoma cell line (OVCAR8) by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The sample was incubated with the primary antibody (1/700 in 0.01M PBS + 1% BSA + 0.5% Triton X-100) for 8 hours at 4°C. ab150097, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1500) was used as the secondary antibody.
Gating Strategy: Isotype control (black line in histogram).
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