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Synthetic peptide within Human p53 aa 277-296 (internal sequence). The exact sequence is proprietary.
(Peptide available as
Our Abpromise guarantee covers the use of ab2433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).Can be blocked with Human p53 peptide (ab2434).|
|ICC/IF||Use a concentration of 1 mg/ml.|
|Flow Cyt||Use at an assay dependent concentration.|
ab2433 staining p53 in human ovarian carcinoma cell line (OVCAR8) by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The sample was incubated with the primary antibody (1/700 in 0.01M PBS + 1% BSA + 0.5% Triton X-100) for 8 hours at 4°C. ab150097, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1500) was used as the secondary antibody.
Gating Strategy: Isotype control (black line in histogram).
ICC/IF image of ab2433 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2433, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L)ab150077) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab2433 shown recognizing mouse and human p53 in Western blot. It was used at a 1/200 dilution for western on A549, MCF-7 human cells and B16F10. Predicted Band 53 kDa.
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