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Recombinant full length protein corresponding to Human p53 (N terminal). epitope is within aa 20-25
Database link: P04637
Dilute in PBS or medium which is identical to that used in the assay system.
Alternative versions available:
Our Abpromise guarantee covers the use of ab1101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.
Use at an assay dependent concentration.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|IP||Use a concentration of 1 - 5 µg/ml.|
|WB||1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 43.6 kDa).|
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: p53 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: A431 cell lysate (20 µg)
Lanes 4, 8 and 12: Saos-2 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab1101 observed at 53 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab181602 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab1101 was shown to specifically react with p53 when p53 knockout samples were used. Wild-type and p53 knockout samples were subjected to SDS-PAGE. ab1101 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
IHC image of ab1101 staining p53 in human colon adenocarcinoma formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1101, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. High magnification of the tumor region - T (lower right panel) and adjacent normal crypts - N (lower left panel) are shown.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Chromatin was prepared from 293 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab1101, and 10ml of protein A sepharose beads,10ml of protein G sepharose beads. No IgG was added to the beads control. The immunoprecipitated DNA was quantified by real time PCR. Primers were as follows:
Bax-1, forward: GGGTTATCTCTTGGGCTCACAA.
Bax-1, reverse: GAGCTCTCCCCAGCGCA.
Bax-2, forward: TGG AGC TGC AGA GGA TGA TTG
Bax-2, reverse: CCA GTT GAA GTT GCC GTC AGA
PUMA, forward: ATG CCT GCC TCA CCT TCA TC
PUMA, reverse: TCA CAC GTC GCT CTC TCT AAA CC
p21-1, forward: GCT GTG GCT CTG ATT GGC TTT
p21-1, reverse: ACA GGC AGC CCA AGG ACA AA
p21-2, forward: CAT CCC CAC AGC AGA GGA GAA
p21-2, reverse: ACC CAG GCT TGG AGC AGC TA
p21-3, forward: GAG TCC TGT TTG CTT CTG GGC A
p21-3, reverse: CTG CAT TGG GGC TGC CTA TGT A
PCNA, forward: CCA CCA TAA AGC TGG GGC TT
PCNA, reverse: TCT CCC CGC CTC TTT GAC TC
ICC/IF image of ab1101 stained human HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1101, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) ab150113 used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and MCF7 cells.
Immunohistochemistry (Frozen sections) using ab1101 (1:100) in human brain tissue.
Overlay histogram showing HeLa cells stained with ab1101 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1101, 1:50 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab1101 overnight at 4°C. Antibody binding was detected using ab175783 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Image courtesy anonymous Abreview
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