Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast cancer cell-lines)
Loading amount 20 µg
Specification Breast cancer cell-lines
Gel Running Conditions Reduced Denaturing (4-20% Nupage gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution 1/2000
Incubation time 1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Milk in TBS-TWEEN

Secondary antibody

Name Non-Abcam antibody was used: Anti-mouse secondary
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/5000

Detection

Detection method ECL+
Exposure 10 second(s)
Bands Specific: 53 kDa Non-specific: 72 kDa
Positive control Mutant MDA231 cell-line

Additional data

Additional Notes 1. MCF7 cells (p53 WT), 2. MDA231 cells (p53 Mutant)
The antibody gives a stronger band at the non-specific 75KDa size but can detect the 53KDa protein well.
Username

Dr. Cherie Blenkiron

Verified customer

Submitted Jul 18 2008