Overview

  • Product nameAnti-p53 BP3 antibody
    See all p53 BP3 primary antibodies
  • Description
    Mouse polyclonal to p53 BP3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Fusion protein:

    NKRSRSRESSSRPRARKDKKRSRTRDSSWSRRAQTLSLSSGSTSRSRSRS SDHGKRRSRSRNRDRYYLRNNYGSKYKWEYTYYSRNKDRDGYESSYRRRT

    , corresponding to amino acids 625/724 of Mouse p53 BP3

  • General notesProduced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab21985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 119 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionFunctions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.
  • Tissue specificityExpressed at highest levels in testis and at lower levels in adrenal gland, bone marrow, brain, colon, heart, kidney, liver, muscle, ovary, pancreas, placenta, prostate, skeletal muscle, skin, small intestine, spleen, stomach, testis, thymus, thyroid and uterus. Expressed in the alveolar epithelium of the lung. Expression is commonly decreased in colon adenocarcinomas and lung cancers.
  • Involvement in diseaseDefects in TOPORS are the cause of retinitis pigmentosa type 31 (RP31) [MIM:609923]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP31 inheritance is autosomal dominant.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Phosphorylation at Ser-98 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity. Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation.
    Sumoylated.
  • Cellular localizationNucleus. Nucleus > PML body. Localizes to discrete nuclear foci which partly overlap with PML nuclear bodies. Targeted to PML nuclear bodies upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW105885 antibody
    • E3 ubiquitin protein ligase Topors antibody
    • E3 ubiquitin-protein ligase Topors antibody
    • LUN antibody
    • p53 binding protein 3 antibody
    • p53 BP3/LUN antibody
    • p53-binding protein 3 antibody
    • p53BP3 antibody
    • p53BP3/LUN antibody
    • Retinitis pigmentosa 31 antibody
    • RP31 antibody
    • SUMO1 protein E3 ligase Topors antibody
    • SUMO1-protein E3 ligase Topors antibody
    • Topoisomerase I binding arginine/serine rich antibody
    • Topoisomerase I binding arginine/serine rich protein antibody
    • Topoisomerase I binding protein antibody
    • Topoisomerase I binding RING finger protein antibody
    • Topoisomerase I-binding arginine/serine-rich protein antibody
    • Topoisomerase I-binding RING finger protein antibody
    • Topoisomerase I-binding RS protein antibody
    • Topors antibody
    • TOPRS_HUMAN antibody
    • TP53BPL antibody
    • Tumor protein p53 binding protein antibody
    • Tumor suppressor p53 binding protein 3 antibody
    • Tumor suppressor p53-binding protein 3 antibody
    see all

Anti-p53 BP3 antibody images

  • All lanes : Anti-p53 BP3 antibody (ab21985) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 119 kDa

References for Anti-p53 BP3 antibody (ab21985)

ab21985 has not yet been referenced specifically in any publications.

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