Overview

  • Product name
  • Description
    Rabbit polyclonal to p53 DINP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human p53 DINP1.

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). Abcam recommends using milk as the blocking agent (3%).

Target

  • Function
    In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis.
  • Tissue specificity
    Ubiquitously expressed.
  • Cellular localization
    Nucleus > PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434M1317 antibody
    • FLJ22139 antibody
    • p53 damage inducible nuclear protein 1 antibody
    • p53 dependent damage inducible nuclear protein 1 antibody
    • p53 inducible nuclear protein 1 antibody
    • p53 inducible p53DINP1 antibody
    • p53-dependent damage-inducible nuclear protein 1 antibody
    • p53DINP1 antibody
    • SIP antibody
    • Stress induced protein antibody
    • Stress-induced protein antibody
    • T53I1_HUMAN antibody
    • Teap antibody
    • TP53 DINP1 antibody
    • TP53 INP1 antibody
    • TP53DINP1 antibody
    • TP53INP1 antibody
    • TP53INP1A antibody
    • TP53INP1B antibody
    • Tumor protein p53 inducible nuclear protein 1 antibody
    • Tumor protein p53-inducible nuclear protein 1 antibody
    see all

Images

  • Anti-p53 DINP1 antibody (ab154877) at 1 µg/ml (Milk blocking - 3%) + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 16 minutes


    Abcam recommends using milk as the blocking agent (3%). This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab154877 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab154877 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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