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Abcam’s Human p53 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of p53 in serum, plasma and cell lysates.
A monoclonal antibody specific p53 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known p53 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for p53 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of p53 present in the samples.
This kit will recognize both endogenous and recombinant Human p53.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated anti p53||Red||1 x 400µl||2 x 400µl|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml||1 x 13ml|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|Control||Silver||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|p53 Microplate (12 x 8 well strips)||1 unit||2 units|
|p53 Standard (Lyophilized)||Yellow||2 vials||4 vials|
|Standard Diluent (Human Serum)||1 x 7ml||2 x 7ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab46067 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"