|
|
|
|
Immunoprecipitation - p53 (mono methyl K372) antibody (ab16033)
(enlarge)
|
|
Immunoprecipitation - p53 (mono methyl K372) antibody (ab16033)
(enlarge)
|
|
Western blot - p53 (mono methyl K372) antibody (ab16033)
(enlarge)
|
|
|
|
|
Product Name
|
p53 (mono methyl K372) antibody
|
| |
See all p53 antibodies (79)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to p53 (mono methyl K372)
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human p53, mono methylated at K372.
(Peptide available as ab16203.)
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
AGMk, Chmp, CynMk, Dog, Mcq, NHuPrm
(Due to sequence homology)
|
|
Tested applications
(see key)
|
IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IP: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 0.05 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 53 kDa).
Note: In vivo (HEK293 cells) this antibody recognizes p53 (monomethyl K372) only through IP-Western. It can detect the target protein in straight WB but only in HEK293 cells with Set7/9 wt. In vitro the antibody works both for IP and in WB. Other applications have not been tested. Blocking with non-methylated peptide (ab16204) should reduce non-specific binding.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic, Endoplasmic reticulum and Nuclear
|
|
Research areas
|
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> p53
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> p53
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> p53
|
|
Relevance
|
p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. p53 is phosphorylated at Ser6 and Ser9 by ck1d and ck1e both in vitro and in vivo. Phosphorylation of p53 at Ser46 is important in regulating the ability of p53 to induce apoptosis. In vivo phosphorylation of p53 at Ser33 by cdk7/cyclin H and in response to UV irradiation has been observed.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 0.10 - 0.80 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this p53 (mono methyl K372) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for p53 (mono methyl K372) antibody images, references, products related to ab16033 and other tools.
|
| |
p53 (mono methyl K372) antibody images:
|
 Immunoprecipitation - p53 (mono methyl K372) antibody (ab16033)
Whole cell extracts derived from HEK293 cells stably expressing either Set7/9 wt or Set7/9 DN mutant were subjected to immunoprecipitation with anti-p53 monomethyl K372 antibody, ab16033. The input and immunoprecipitated materials were resolved by SDS-PAGE and analyzed by immunoblotting using p53-specific antibody (Ab6-Oncogene). Lanes 1 and 3 represent 2% of input materials for Set7/9wt and Set7/9DN mutant cell lines, respectively.
Lanes 2 and 4 represent immunoprecipitated materials from Set7/9wt and Set7/9DN extracts, respectively. The data shows that p53 monomethyl K372 was succssfully IPed from the Set7/9 wt but not the Set 7/9 DN mutant cells.
NB:
The antibody was exhausted twice with 5 ug of control p53 peptide each time to eliminate non-specific binding to unmethylated p53. For westerns 5 ug of the blocking peptide was used per 10 ml of p53-K372me1 antibody in 1:5000 dilution. Western blots were blocked in PBS with 2% non-fat milk for 2 hrs following the overnight incubation with p53-K372 me1-specific antibody (1:5000 and 5 ug of the blocking peptide). Secondary goat anti-rabbit antibody conjugated to peroxidase (BioRad) was used followed by incubation with ECL to develop the signal. T. Ivanova, Tufts University
|
| |
Immunoprecipitation - p53 (mono methyl K372) antibody (ab16033)
WB and IP using ab16033
In vitro methylated or mock-methylated GST-p53 fragments (fragment = AAs300-393) were subjected to immunoprecipitation with anti-p53 monomethyl K372 antibody, ab16033. (A) Western blotting with GST-specific antibody.
Lanes 1 and 2 – input materials representing 20% of methylated and mock-methylated GST-p53, respectively.
Lanes 3 and 4 – immunoprecipitated methylated and mock-methylated p53 proteins. ab16033 was able to IP methylated but not unmethylated (mock methylated) GST-p53 fragments. (B) Western blotting with anti-p53 monomethyl K372 antibody, ab16033.
Lanes 1 and 2 – input materials representing 20% of methylated and mock-methylated GST-p53, respectively.
Lanes 3 and 4 – immunoprecipitated methylated and mock-methylated p53 proteins. ab16033 was able to IP and recognise by Western Blot methylated but not unmethylated (mock methylated) GST-p53 fragments.
NB:
The antibody was exhausted twice with 5 ug of control p53 peptide each time to eliminate non-specific binding to unmethylated p53. For westerns 5 ug of the blocking peptide was used per 10 ml of p53-K372me1 antibody in 1:5000 dilution. Western blots were blocked in PBS with 2% non-fat milk for 2 hrs following the overnight incubation with p53-K372 me1-specific antibody (1:5000 and 5 ug of the blocking peptide). Secondary goat anti-rabbit antibody conjugated to peroxidase (BioRad) was used followed by incubation with ECL to develop the signal. T. Ivanova, Tufts University
|
| |
|
|
| |
References for p53 (mono methyl K372) antibody (ab16033)
|
|
This product has been used in:
Ivanov GS et al. Methylation-acetylation interplay activates p53 in response to DNA damage. Mol Cell Biol 27:6756-69 (2007). WB; Human. PubMed: 17646389
If you publish research using ab16033 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to p53
|
| |
p53 (mono methyl K372) antibody - more information
|
Customer reviews (feedback) regarding p53 (mono methyl K372) antibody |
Customer FAQs regarding p53 (mono methyl K372) antibody |
Protocols for p53 (mono methyl K372) antibody |
Price and availability of products related to p53 (mono methyl K372) antibody |
| |
p53 (mono methyl K372) antibody - related products:
|
|
|
| |
p53 (mono methyl K372) antibody - other tools:
|
Contact Abcam with a Technical Enquiry about p53 (mono methyl K372) antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
