|MCF7 cells||< 10%|
ab135393 is an In-Cell ELISA (ICE) assay kit that uses quantitative immunocytochemistry to measure levels of p53 phosphorylated at Ser9 and total p53 protein levels in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized antibodies. Relative target levels are quantified using IRDye®-labeled Secondary Antibodies and an IR imaging system.
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
|Components||1 x 96 tests|
|1000X IRDye® Labeled Secondary Antibody Cocktail||1 x 24µl|
|100X Mouse Anti-p53 Primary Antibody||1 x 120µl|
|100X Rabbit Anti-p53 pSer9 Antibody||1 x 120µl|
|100X Triton X-100||1 x 500µl|
|10X Blocking Solution||1 x 10ml|
|10X Buffer||1 x 10ml|
|10X Phosphate Buffered Saline||1 x 100ml|
|1X Janus Green Stain||1 x 11ml|
|400X Tween-20||1 x 2ml|
Our Abpromise guarantee covers the use of ab135393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||Use at an assay dependent concentration.|
ab135393 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"