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Product name
Anti-p53R2 antibody
See all p53R2 products (3) ...
Description
Rabbit polyclonal to p53R2
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: GDPERPEAAGLDQDER, corresponding to amino acids 2-17 of Human p53R2.
GDPERPEAAGLDQDER
Positive control
A431 or 3T3 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 18997010).
IHC-P: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
Tissue specificity
Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
Pathway
Genetic information processing; DNA replication.
Involvement in disease
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase small chain family.
Cellular localization
Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
Target information above from: UniProt accessionQ7LG56
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686M05248 antibody
- MGC102856 antibody
- MGC42116 antibody
see all
Database links
- Entrez Gene: 50484 Human
- Entrez Gene: 382985 Mouse
- Omim: 604712 Human
- SwissProt: Q9NTD8 Human
- SwissProt: Q7LG56 Human
- SwissProt: Q6PEE3 Mouse
- Unigene: 512592 Human
- Unigene: 24738 Mouse
Anti-p53R2 antibody images:
Western blot - p53R2 antibody (ab8105)
Anti-p53R2 antibody (ab8105) at 1/1000 dilution + U2OS whole cell lysate treated with UV irradiation.
Secondary
Goat anti-rabbit at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 41 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa (possible cross reactivity).
Exposure time : 1 minute
Blot was incubated for one hour in 5% milk for blocking, and incubated in primary antibody for 16 hours.
Image was taken from an anonymously submitted Abreview
Western blot - p53R2 antibody (ab8105)
All lanes : Anti-p53R2 antibody (ab8105) at 1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : 3T3 cell lysate with absence of blocking peptide
Lane 3 : 3T3 cell lysate with presence of blocking peptide
Predicted band size : 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p53R2 antibody (ab8105)
ab8105 at 1µg/ml staining p53R2 in lung tissue by IHC
Immunocytochemistry/ Immunofluorescence - p53R2 antibody (ab8105)
ICC/IF image of ab8105 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8105, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-p53R2 antibody (ab8105)
This product has been referenced in:
- Mansson E et al. Down-regulation of deoxycytidine kinase in human leukemic cell lines resistant to cladribine and clofarabine and increased ribonucleotide reductase activity contributes to fludarabine resistance. Biochem Pharmacol 65:237-47 (2003).Read more (PubMed: 12504799) »
- Pontarin G et al. Deoxyribonucleotide Metabolism in Cycling and Resting Human Fibroblasts with a Missense Mutation in p53R2, a Subunit of Ribonucleotide Reductase. J Biol Chem 286:11132-40 (2011). WB; Human.Read more (PubMed: 21297166) »
See all 8 publications for this product
Publishing research using ab8105? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"