Validated using a knockout cell line
Recombinant
RabMAb

Anti-p53R2 antibody [EPR8816] (ab154194)

Overview

  • Product name
    Anti-p53R2 antibody [EPR8816]
    See all p53R2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8816] to p53R2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human p53R2 (N terminal).

  • Positive control
    • Human fetal muscle tissue, MCF7 and SW480 lysates; MCF7 cells; Human muscle and ovarian carcinoma tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab154194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
  • Tissue specificity
    Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
  • Pathway
    Genetic information processing; DNA replication.
  • Involvement in disease
    Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A) [MIM:612075]. A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy.
    Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B) [MIM:612075]. A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy.
    Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5) [MIM:613077]. A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
  • Sequence similarities
    Belongs to the ribonucleoside diphosphate reductase small chain family.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates from cytoplasm to nucleus in response to DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686M05248 antibody
    • MGC102856 antibody
    • MGC42116 antibody
    • MTDPS8A antibody
    • MTDPS8B antibody
    • p53 inducible ribonucleotide reductase small subunit 2 homolog antibody
    • p53 inducible ribonucleotide reductase small subunit 2 like protein antibody
    • P53 inducible ribonucleotide reductase small subunit 2 short form beta antibody
    • p53 R2 antibody
    • p53-inducible ribonucleotide reductase small subunit 2-like protein antibody
    • p53R2 antibody
    • Ribonucleoside diphosphate reductase M2 subunit B antibody
    • Ribonucleoside-diphosphate reductase subunit M2 B antibody
    • Ribonucleotide reductase M2 B (TP53 inducible) antibody
    • Ribonucleotide reductase M2 B antibody
    • Ribonucleotide reductase small subunit like 2 p53 inducible antibody
    • RIR2B_HUMAN antibody
    • RRM 2B antibody
    • RRM2B antibody
    • TP53 inducible ribonucleotide reductase M2 B antibody
    • TP53-inducible ribonucleotide reductase M2 B antibody
    see all

Images



  • Predicted band size : 40 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: p53R2 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: SW480 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab154194 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
    ab154194 was shown to recognize p53R2 when p53R2 knockout samples were used, along with additional cross-reactive bands. Wild-type and p53R2 knockout samples were subjected to SDS-PAGE. ab154194 and ab18058 (loading control to Vinculin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154194 showing +ve staining.

  • Immunofluorescent staining of MCF7 cells labeling p53R2 with ab154194 at 1/250 dilution
  • Flow cytometric analysis of permeabilized MCF7 cells labeling p53R2 with ab154194 at 1/10 dilution (red) or a rabbit IgG negative control antibody (green).
  • All lanes : Anti-p53R2 antibody [EPR8816] (ab154194) at 1/1000 dilution

    Lane 1 : Human fetal muscle lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 40 kDa
  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling p53R2 with ab154194 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin embedded Human melanoma tissue using ab154194 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154194 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154194 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154194 showing +ve staining.

References

ab154194 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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