Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-p55/DCAF1 antibody
See all p55/DCAF1 products (2) ...
Description
Rabbit polyclonal to p55/DCAF1
Tested applications
IF, IP, WBmore details
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Predicted to work with
Chicken, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: CNVYNDEEPEIPASELETNTA, corresponding to amino acids 411-430 of Drosophilia melanogaster CAF 1 (DCAF 1).
CNVYNDEEPEIPASELETNTA
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.02% sodium azide
Purity
Whole antiserum
Primary antibody notes
Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 48.6 kDa.
Target
Relevance
p55/DCAF1 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair.
Cellular localization
Nuclear
Alternative names
- Caf 1 antibody
- CAF-1 P55 subunit antibody
- CAF1 p55 subunit antibody
see all
Database links
- Entrez Gene: 41836 Fruit fly (Drosophila melanogaster)
- Entrez Gene: 779086 Xenopus laevis
- SwissProt: Q24572 Fruit fly (Drosophila melanogaster)
- SwissProt: Q07CZ6 Xenopus laevis
References for Anti-p55/DCAF1 antibody (ab1766)
This product has been referenced in:
- Lee H et al. Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. Mol Cell Biol 30:2563-77 (2010). WB; Fruit fly (Drosophila melanogaster).Read more (PubMed: 20176807) »
- Groth A et al. Human Asf1 regulates the flow of S phase histones during replicational stress. Mol Cell 17:301-11 (2005).Read more (PubMed: 15664198) »
See all 2 publications for this product
Publishing research using ab1766? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"