Overview

  • Product name
    Anti-p55/DCAF1 antibody - ChIP Grade
    See all p55/DCAF1 primary antibodies
  • Description
    Rabbit polyclonal to p55/DCAF1 - ChIP Grade
  • Tested applications
    Suitable for: ChIP, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    CNVYNDEEPEIPASELETNTA

    , corresponding to amino acids 411-430 of Drosophilia melanogaster CAF 1 (DCAF 1).

  • General notes


    Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.

Properties

Applications

Our Abpromise guarantee covers the use of ab1766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP 1/450.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Target

  • Relevance
    p55/DCAF1 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Caf 1 antibody
      • CAF1 p55 subunit antibody
      • chaf1b antibody
      • Chromatin assembly factor 1 p55 subunit antibody
      • Chromatin assembly factor 1 p60 subunit antibody
      • chromatin assembly factor 1 subunit antibody
      • dCAF1 antibody
      • NURF 55 antibody
      • p55 antibody
      • Probable histone binding protein Caf1 antibody
      see all

    Images

    • Chromatin was obtained from Kc167 cells fixed with formaldehyde for 10 min. The ChIP was performed with 30-40 µg of chromatin, ab1766 at 1/450 dilution (incubated with samples for 16h at 4°C) and 20µl of Protein G Agarose beads per ChIP. The immunoprecipitated DNA was quantified by real time PCR (SYBR) . Gene A's enhancer region locus (positive control) shows enrichment when compared with it's coding sequence locus (negative control).

      See Abreview

    • All lanes : Anti-p55/DCAF1 antibody - ChIP Grade (ab1766) at 1/2000 dilution

      Lane 1 : Kc167 cell lysate
      Lane 2 : DmD8 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG (H+L)-HRP Conjugate at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 55 kDa
      Observed band size : 55 kDa


      Exposure time : 1 minute

      This image is courtesy of an Abreview submitted by Dr Kat Millen

      See Abreview

    References

    This product has been referenced in:
    • Lee H  et al. A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes. Mol Cell Biol 32:2110-20 (2012). IP, WB ; Drosophila melanogaster . Read more (PubMed: 22451490) »
    • Lee H  et al. Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. Mol Cell Biol 30:2563-77 (2010). WB ; Drosophila melanogaster . Read more (PubMed: 20176807) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - nuclear (Kc167 cell line)
    Specification
    Kc167 cell line
    Negative control
    Coding sequences ChIP.
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Positive control
    Gene A enhancer region ChIP.
    Username

    Dr. Kat Millen

    Verified customer

    Submitted Jul 25 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Non-reduced Denaturing (4% gel)
    Sample
    Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (DmD8 and Kc167 cell lines)
    Specification
    DmD8 and Kc167 cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Dr. Kat Millen

    Verified customer

    Submitted Jul 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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