Anti-p55/DCAF1 antibody (ab1766)
Key features and details
- Rabbit polyclonal to p55/DCAF1
- Isotype: IgG
Overview
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Product name
Anti-p55/DCAF1 antibody -
Description
Rabbit polyclonal to p55/DCAF1 -
Host species
Rabbit -
Species reactivity
Predicted to work with: Chicken, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
Complex that assembles histone octamers onto replicating DNA in vitro. Caf-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones h3 and h4 to replicating DNA; histones H2a/H2b can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P150 and p60 form complexes with newly synthesized histones h3 and acetylated H4 in cell extracts (by similarity). P55 associates with acetyltransferases and deacetylases in cell extracts and is also a component of the nucleosome remodeling factor complex (NURF), a protein complex consisting of four polypeptides that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. This subunit may assist targeting of protein complexes to chromatin. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Target
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Relevance
p55/DCAF1 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 779086 Xenopus laevis
- SwissProt: Q07CZ6 Xenopus laevis
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Alternative names
- Caf 1 antibody
- CAF1 p55 subunit antibody
- chaf1b antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab1766 has been referenced in 8 publications.
- Zhou Z et al. Merlin functions as a critical regulator in Staphylococcus aureus-induced osteomyelitis. J Cell Physiol 237:815-823 (2022). PubMed: 34378805
- Lattao R et al. Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins. Dev Cell N/A:N/A (2021). PubMed: 33725482
- Zong Y et al. Ddb1-Cullin4-Associated-Factor 1 in Macrophages Restricts the Staphylococcus aureus-Induced Osteomyelitis. J Inflamm Res 14:1667-1676 (2021). PubMed: 33953594
- Kotov AA et al. The Drosophila RNA Helicase Belle (DDX3) Non-Autonomously Suppresses Germline Tumorigenesis Via Regulation of a Specific mRNA Set. Cells 9:N/A (2020). PubMed: 32111103
- Abascal F et al. Subfunctionalization via adaptive evolution influenced by genomic context: the case of histone chaperones ASF1a and ASF1b. Mol Biol Evol 30:1853-66 (2013). PubMed: 23645555
- Lee H et al. A dual role for the dREAM/MMB complex in the regulation of differentiation-specific E2F/RB target genes. Mol Cell Biol 32:2110-20 (2012). IP, WB ; Drosophila melanogaster . PubMed: 22451490
- Lee H et al. Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. Mol Cell Biol 30:2563-77 (2010). WB ; Drosophila melanogaster . PubMed: 20176807
- Groth A et al. Human Asf1 regulates the flow of S phase histones during replicational stress. Mol Cell 17:301-11 (2005). PubMed: 15664198