Overview

  • Product name
  • Description
    Rabbit polyclonal to p57 Kip2
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    (C)GVGSVEQTPRKRLR

    conjugated to KLH, corresponding to amino acids 303-316 of Human p57 Kip2

  • Positive control
    • WB and IP: HeLa nuclear extract and a bovine brain nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab33169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IP
WB
  • Application notes
    ICC/IF: Use at an assay dependent dilution(PMID-19481073)
    IP: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 32 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
    • Tissue specificity
      Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
    • Involvement in disease
      Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
      Note=Defects in CDKN1C are involved in tumor formation.
    • Sequence similarities
      Belongs to the CDI family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beckwith Wiedemann syndrome antibody
      • BWCR antibody
      • BWS antibody
      • CDKI antibody
      • CDKN 1C antibody
      • CDKN1C antibody
      • CDN1C_HUMAN antibody
      • Cyclin dependent kinase inhibitor 1C antibody
      • Cyclin dependent kinase inhibitor p57 antibody
      • Cyclin-dependent kinase inhibitor 1C antibody
      • Cyclin-dependent kinase inhibitor p57 antibody
      • KIP 2 antibody
      • KIP2 antibody
      • p57 antibody
      • p57 Kip 2 antibody
      • p57KIP2 antibody
      • WBS antibody
      see all

    References

    This product has been referenced in:
    • Saravanamuthu SS  et al. Notch signaling is required for lateral induction of Jagged1 during FGF-induced lens fiber differentiation. Dev Biol 332:166-76 (2009). WB, ICC/IF ; Rat . Read more (PubMed: 19481073) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse lung)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Treatment
    mouse lung after 24-hour ischemia
    Specification
    mouse lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 14 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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