Overview

  • Product nameAnti-p57 Kip2 antibody
    See all p57 Kip2 primary antibodies
  • Description
    Rabbit polyclonal to p57 Kip2
  • SpecificityThis antibody reacts with a 57 kDa protein.
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide (Human).

  • Positive control
    • Colon Carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab4058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 17709399
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionPotent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
  • Tissue specificityExpressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
  • Involvement in diseaseDefects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
    Note=Defects in CDKN1C are involved in tumor formation.
  • Sequence similaritiesBelongs to the CDI family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beckwith Wiedemann syndrome antibody
    • BWCR antibody
    • BWS antibody
    • CDKI antibody
    • CDKN 1C antibody
    • CDKN1C antibody
    • CDN1C_HUMAN antibody
    • Cyclin dependent kinase inhibitor 1C antibody
    • Cyclin dependent kinase inhibitor p57 antibody
    • Cyclin-dependent kinase inhibitor 1C antibody
    • Cyclin-dependent kinase inhibitor p57 antibody
    • KIP 2 antibody
    • KIP2 antibody
    • p57 antibody
    • p57 Kip 2 antibody
    • p57KIP2 antibody
    • WBS antibody
    see all

Anti-p57 Kip2 antibody images

  • Tissues were from mouse E14.5 wild-type embryonic heads fixed in neutral buffered formalin sectioned (5-7 um) horizontally through the eyes. Antigen retrieval (0.1M citrate buffer pH6.5) in a 1400W microwave (5 min high; 5 min low). Sections blocked in 0.4% BSA/5% goat serum in PBS for 1h. Antibody diluted (1:100) in block soln, applied overnight at 4C. Detection with anti-Rb Alexa green Ab (1:500), nuclear counterstain with Hoechst dye (bisbenzimide).
  • ab4058 staining mouse E14.5 embryonic eye tissue sections by IHC-Fr.  Sections were PFA fixed and blocked with 1% BSA for 2 hours at 25°C prior to incubating with ab4058, diluted 1/100, for 12 hours at 25°C.  A FITC conjugated goat anti-rabbit antibody, diluted 1/400 was used as the secondary.

    See Abreview

  • ab4958 staining p57 Kip2 in non-transgenic mouse embryos by Immunohistochemistry (PFA fixed, paraffin embedded sections).
    Heads of mice were removed, fixed in 10% formalin, dehydrated, embedded in paraffin, sectioned (5-7 µm).

    Slides containing ocular sections from non-transgenic embryos were first deparaffinized and rehydrated. Antigens were retrieved by microwave treatment in 10 mM Sodium Citrate buffer (pH 6.0). Following antigen retrieval, the tissue sections were blocked with 10% normal horse serum for 30 minutes, at room temperature. The slides were then incubated with ab4058 at a 1/250 dilution overnight at 4°C. Following brief washes in PBS, the slides were incubated with the appropriate biotinylated-secondary antibodies for 30 minutes at 37°C. Antigen-antibody complexes were then detected using streptavidin-linked Alexa 594 (red) at 1/1000 dilution. Sections were mounted using anti-fade media containing DAPI (blue). Images were captured using a Nikon Eclipse
  • Anti-p57 Kip2 antibody (ab4058) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,51 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining p57 Kip2 with ab4058.

References for Anti-p57 Kip2 antibody (ab4058)

This product has been referenced in:
  • Jia H  et al. p57Kip2 is an unrecognized DNA damage response effector molecule that functions in tumor suppression and chemoresistance. Oncogene N/A:N/A (2014). Read more (PubMed: 25195859) »
  • Chaffee BR  et al. Nuclear removal during terminal lens fiber cell differentiation requires CDK1 activity: appropriating mitosis-related nuclear disassembly. Development 141:3388-98 (2014). Read more (PubMed: 25139855) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat Placenta)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Rat Placenta
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative Formaldehyde
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Verified customer

Submitted Mar 26 2016

Thank you for contacting us.

We recommend that alignment should be over 85% to predict that an antibody will detect in a different species. The identity between the immunogen and the danio protein is low at 47%. I would therefore suggest this ...

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Thank you for your note.

This is to confirm that the free of charge replacement was dispatched last week and the item has probably arrived in Australia. I would suggest contacting Sapphire to get some information about the delivery time.
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Thank you for your e-mail.

This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab4058 (from a different batch) as a free of charge replacement. The new order number is 1204023. <...

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Thank you for your enquiry regarding ab4058 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

It is very clear from the attached images that the...

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I am very sorry to hear that you have been experiencing problems using our products (ab4058, ab38686, ab72130, ab80645) in the application that you wish.

As our Abpromise indicates, in the event that a product is not functioning in the applic...

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Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement from the older lot with the order number.
<...

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We are always happy to help.
I wish you the best of luck for your research.
Have a great day!

Thank you very much for contacting us and for your patience. I apologise for the delay.
The originator of this antibody confirmed that the immunogen is a proprietary sequence located at the C-terminus of human p57 Kip2. Our BLAST searches revealed...

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Thank you very much for your consultation. It is rather difficult to compare the antibodies for effectiveness. Please note that the application notes on the datasheet are only an indication and can vary depending on the laboratory and the settings. The...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"