Anti-p57 Kip2 antibody [KP39] (ab3223)

Overview

  • Product nameAnti-p57 Kip2 antibody [KP39]
    See all p57 Kip2 primary antibodies
  • Description
    Mouse monoclonal [KP39] to p57 Kip2
  • Tested applicationsSuitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • LS174T cells. Colon carcinomas or placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab3223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2 µg/mg of lysate. Native.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionPotent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.
    • Tissue specificityExpressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.
    • Involvement in diseaseDefects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.
      Note=Defects in CDKN1C are involved in tumor formation.
    • Sequence similaritiesBelongs to the CDI family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beckwith Wiedemann syndrome antibody
      • BWCR antibody
      • BWS antibody
      • CDKI antibody
      • CDKN 1C antibody
      • CDKN1C antibody
      • CDN1C_HUMAN antibody
      • Cyclin dependent kinase inhibitor 1C antibody
      • Cyclin dependent kinase inhibitor p57 antibody
      • Cyclin-dependent kinase inhibitor 1C antibody
      • Cyclin-dependent kinase inhibitor p57 antibody
      • KIP 2 antibody
      • KIP2 antibody
      • p57 antibody
      • p57 Kip 2 antibody
      • p57KIP2 antibody
      • WBS antibody
      see all

    References for Anti-p57 Kip2 antibody [KP39] (ab3223)

    ab3223 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.

    Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted an...

    Read More

    Thank you for your patience. I regret to inform you that this antibody, ab3223, is NOT suitable for application in Western blotting. This information was missing on our datasheet and the online datasheet has now been updated to include this. I apol...

    Read More

    I'm sorry to hear that you are still experiencing trouble with this antibody. Your blots are completely blank? What is the batch number that you received (it's located on the vial)? What is the Abcam order number or purchase order number used?

    I am sorry to hear that you are experiencing trouble with this antibody and also, thank you for your patience. What dilutions have you tried using ab3223 at? At this point my suggestion is to increase the concentration of the primary antibody - try 1:1...

    Read More

    Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

    Read More

    We do not have the sequence of this protein Recombinant protein was used to develop this antibody.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"