Overview

  • Product nameAnti-p60 CAF1 antibody [SS 53]See all p60 CAF1 primary antibodies ...
  • Description
    Mouse monoclonal [SS 53] to p60 CAF1
  • Specificityspecific for CAF-1p60.
  • Tested applicationsWB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • General notes


    Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferAscitic fluid with 0.1% sodium azide
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notes Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.
  • Clonality Monoclonal
  • Clone numberSS 53
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IP
IHC-P
  • Application notesICC/IF: 1/800.
    IHC-P: Use at an assay dependent dilution (PMID 19309489).
    IP: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 60 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComplex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
    • Sequence similaritiesBelongs to the WD repeat HIR1 family.
      Contains 7 WD repeats.
    • Developmental stageActive complex is found in G1, S and G2 phases.
    • Post-translational
      modifications
      Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
    • Cellular localizationNucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAF 1 subunit B antibody
      • CAF 1A antibody
      • CAF I 60 kDa subunit antibody
      • CAF IP60 antibody
      • CAF-1 antibody
      • CAF-1 antibody
      • CAF-1 subunit B antibody
      • CAF-I 60 kDa subunit antibody
      • CAF-I p60 antibody
      • CAF1 antibody
      • CAF1A antibody
      • CAF1B_HUMAN antibody
      • CAF1P60 antibody
      • CHAF 1B antibody
      • CHAF1B antibody
      • Chromatin assembly factor 1 subunit B antibody
      • Chromatin assembly factor I antibody
      • Chromatin assembly factor I antibody
      • Chromatin assembly factor I p60 subunit antibody
      • Human chromatin assembly factor-I p60 subunit antibody
      • M phase phosphoprotein 7 antibody
      • M-phase phosphoprotein 7 antibody
      • MPHOSPH7 antibody
      • MPP 7 antibody
      • MPP7 antibody
      • p60 subunit antibody
      • p60 subunit antibody
      see all

    Anti-p60 CAF1 antibody [SS 53] images

    • ab8133 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/800 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-mouse antibody diluted 1/400 was used as the secondary.

      See Abreview

    References for Anti-p60 CAF1 antibody [SS 53] (ab8133)

    This product has been referenced in:
    • Oh J  et al. Chromatin assembly on herpes simplex virus 1 DNA early during a lytic infection is Asf1a dependent. J Virol 86:12313-21 (2012). Read more (PubMed: 22951827) »
    • Zhu Q  et al. Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites. DNA Repair (Amst) 8:262-73 (2009). Read more (PubMed: 19059499) »

    See all 7 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse Neuroblastoma cell line N2A)
    Loading amount 30 µg
    Specification Mouse Neuroblastoma cell line N2A
    Gel Running Conditions Reduced Denaturing (13%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 16 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 40 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (9)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 5°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 29 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein (Recombinant from insect cells)
    Loading amount 0.02 µg
    Specification Recombinant from insect cells
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 14 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (HeLa cells)
    Specification HeLa cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Alexander Rapp

    Verified customer

    Submitted Apr 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"