Overview

  • Product name
  • Description
    Rabbit polyclonal to p60 CAF1
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 375 and 425 of human p60 CAF1 (NP_005432.1)

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
    • Sequence similarities
      Belongs to the WD repeat HIR1 family.
      Contains 7 WD repeats.
    • Developmental stage
      Active complex is found in G1, S and G2 phases.
    • Post-translational
      modifications
      Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
    • Cellular localization
      Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAF 1 subunit B antibody
      • CAF 1A antibody
      • CAF I 60 kDa subunit antibody
      • CAF IP60 antibody
      • CAF-1 antibody
      • CAF-1 subunit B antibody
      • CAF-I 60 kDa subunit antibody
      • CAF-I p60 antibody
      • CAF1 antibody
      • CAF1A antibody
      • CAF1B_HUMAN antibody
      • CAF1P60 antibody
      • CHAF 1B antibody
      • CHAF1B antibody
      • Chromatin assembly factor 1 subunit B antibody
      • Chromatin assembly factor I antibody
      • Chromatin assembly factor I p60 subunit antibody
      • Human chromatin assembly factor-I p60 subunit antibody
      • M phase phosphoprotein 7 antibody
      • M-phase phosphoprotein 7 antibody
      • MPHOSPH7 antibody
      • MPP 7 antibody
      • MPP7 antibody
      • p60 subunit antibody
      see all

    Images

    • Immunoprecipitation/ Western Blot of p60 CAF1.
      Lane 1: ab72519 at 3µg/mg Hela whole cell lysate (at 1mg for IP, 20% of IP loaded)
      Lane 2: Control IgG.

      A different antibody was used for the WB.

    References

    ab72519 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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