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Synthetic peptide from a region between residue 509 and the C terminus (residue 559) of human p60 CAF1 (NP_005432.1)
Our Abpromise guarantee covers the use of ab72520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
ab72520 staining p60 CAF1 in human PC3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF568-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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