Anti-p60 CAF1/MPP7 antibody (ab72520)
Key features and details
- Rabbit polyclonal to p60 CAF1/MPP7
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-p60 CAF1/MPP7 antibody
See all p60 CAF1/MPP7 primary antibodies -
Description
Rabbit polyclonal to p60 CAF1/MPP7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human p60 CAF1/MPP7 (C terminal).
Database link: NP_005432.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa).
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
Notes |
---|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
-
Function
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex. -
Sequence similarities
Belongs to the WD repeat HIR1 family.
Contains 7 WD repeats. -
Developmental stage
Active complex is found in G1, S and G2 phases. -
Post-translational
modificationsDifferentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases. -
Cellular localization
Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase. - Information by UniProt
-
Database links
- Entrez Gene: 8208 Human
- Omim: 601245 Human
- SwissProt: Q13112 Human
- Unigene: 75238 Human
-
Alternative names
- CAF 1 subunit B antibody
- CAF 1A antibody
- CAF I 60 kDa subunit antibody
see all
Images
-
All lanes : Anti-p60 CAF1/MPP7 antibody (ab72520) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 240 kDa, 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling p60 CAF1/MPP7 with ab7252 at 1/200 (1µg/ml). Detection: DAB.
-
Immunoprecipitation/ Western Blot of p60 CAF1/MPP7. Lane 1: ab72520 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72520 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab72520 has been referenced in 2 publications.
- Gong L et al. Ubiquitin ligase CHAF1B induces cisplatin resistance in lung adenocarcinoma by promoting NCOR2 degradation. Cancer Cell Int 20:194 (2020). PubMed: 32508530
- Yang C et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res 45:739-748 (2017). PubMed: 27794043