The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa).
Use at 2-5 µg/mg of lysate.
FunctionComplex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
Sequence similaritiesBelongs to the WD repeat HIR1 family. Contains 7 WD repeats.
Developmental stageActive complex is found in G1, S and G2 phases.
Post-translational modificationsDifferentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Cellular localizationNucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling p60 CAF1 with ab7252 at 1/200 (1µg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-p60 CAF1 antibody (ab72520)This image is courtesy of an Abreview submitted by Dimitra Kalamida
ab72520 staining p60 CAF1 in human PC3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF568-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
All lanes : Anti-p60 CAF1 antibody (ab72520) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of p60 CAF1.
Lane 1: ab72520 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72520 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
References for Anti-p60 CAF1 antibody (ab72520)
has not yet been referenced specifically in any publications.
Publishing research using ab72520? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"