Overview

  • Product nameAnti-p60 CAF1 antibody
    See all p60 CAF1 primary antibodies
  • Description
    Rabbit polyclonal to p60 CAF1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 509 and the C terminus (residue 559) of human p60 CAF1 (NP_005432.1)

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab72520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 25, 65, 130, 240 kDa (predicted molecular weight: 61 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionComplex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
  • Sequence similaritiesBelongs to the WD repeat HIR1 family.
    Contains 7 WD repeats.
  • Developmental stageActive complex is found in G1, S and G2 phases.
  • Post-translational
    modifications
    Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
  • Cellular localizationNucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAF 1 subunit B antibody
    • CAF 1A antibody
    • CAF I 60 kDa subunit antibody
    • CAF IP60 antibody
    • CAF-1 antibody
    • CAF-1 subunit B antibody
    • CAF-I 60 kDa subunit antibody
    • CAF-I p60 antibody
    • CAF1 antibody
    • CAF1A antibody
    • CAF1B_HUMAN antibody
    • CAF1P60 antibody
    • CHAF 1B antibody
    • CHAF1B antibody
    • Chromatin assembly factor 1 subunit B antibody
    • Chromatin assembly factor I antibody
    • Chromatin assembly factor I p60 subunit antibody
    • Human chromatin assembly factor-I p60 subunit antibody
    • M phase phosphoprotein 7 antibody
    • M-phase phosphoprotein 7 antibody
    • MPHOSPH7 antibody
    • MPP 7 antibody
    • MPP7 antibody
    • p60 subunit antibody
    see all

Anti-p60 CAF1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling p60 CAF1 with ab7252 at 1/200 (1µg/ml). Detection: DAB.
  • ab72520 staining p60 CAF1 in human PC3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF568-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-p60 CAF1 antibody (ab72520) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa,240 kDa,25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds
  • Immunoprecipitation/ Western Blot of p60 CAF1.
    Lane 1: ab72520 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72520 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 3 seconds.

References for Anti-p60 CAF1 antibody (ab72520)

ab72520 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (PC3 Prostate cancer cells)
Specification PC3 Prostate cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Jan 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"