Recombinant
RabMAb

Anti-p60 CAF1 antibody [EPR6105] (ab109442)

Overview

  • Product name
    Anti-p60 CAF1 antibody [EPR6105]
    See all p60 CAF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6105] to p60 CAF1
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    A synthetic peptide corresponding to residues at the C-terminus of Human p60 CAF1.

  • Positive control
    • Jurkat, HeLa, 293T, and Caco2 cell lysates; Human colon and tonsil tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6105
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
  • Sequence similarities
    Belongs to the WD repeat HIR1 family.
    Contains 7 WD repeats.
  • Developmental stage
    Active complex is found in G1, S and G2 phases.
  • Post-translational
    modifications
    Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
  • Cellular localization
    Nucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAF 1 subunit B antibody
    • CAF 1A antibody
    • CAF I 60 kDa subunit antibody
    • CAF IP60 antibody
    • CAF-1 antibody
    • CAF-1 subunit B antibody
    • CAF-I 60 kDa subunit antibody
    • CAF-I p60 antibody
    • CAF1 antibody
    • CAF1A antibody
    • CAF1B_HUMAN antibody
    • CAF1P60 antibody
    • CHAF 1B antibody
    • CHAF1B antibody
    • Chromatin assembly factor 1 subunit B antibody
    • Chromatin assembly factor I antibody
    • Chromatin assembly factor I p60 subunit antibody
    • Human chromatin assembly factor-I p60 subunit antibody
    • M phase phosphoprotein 7 antibody
    • M-phase phosphoprotein 7 antibody
    • MPHOSPH7 antibody
    • MPP 7 antibody
    • MPP7 antibody
    • p60 subunit antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-p60 CAF1 antibody [EPR6105] (ab109442)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Caco2 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab109442 at 1/250 dilution staining p60 CAF1 in paraffin-embedded Human colon tissue.
  • ab109442 at 1/250 dilution staining p60 CAF1 in paraffin-embedded Human tonsil tissue.

References

ab109442 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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