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Recombinant fragment corresponding to Human p63 aa 1-205. This antibody is raised against the N-terminus of ∆N p63 isoform of mouse protein.
Database link: Q9H3D4-2
Please note that clone [BC4A4] and clone [4A4] are identical.
This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR323232, GR293168, GR301030]. Lot numbers higher than [GR323232] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 15800938|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
See Sanderson et al and Huange et al. or the Abreview 22860.
Alternatively, steam tissue sections for 45-60 minutes. Allow solution to cool for 10 minutes then wash in distilled water.
|IHC-Fr||Use at an assay dependent concentration.|
Immunohistochemical analysis of prostate tissue labelling p63 with ab735.