Overview

  • Product name
  • Description
    Rabbit polyclonal to p66 beta
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 543 and 593 of human p66 beta (NP_065750.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Contains 1 GATA-type zinc finger.
    • Domain
      CR1 is required for interaction with MBD3 and transcription repression.
      CR2 is required for localization to discrete loci in the nucleus.
    • Cellular localization
      Nucleus. Nuclear, in discrete foci.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ37346 antibody
      • GATA zinc finger domain containing 2B antibody
      • GATA zinc finger domain containing protein 2B antibody
      • GATA zinc finger domain-containing protein 2B antibody
      • GATAD2B antibody
      • KIAA1150 antibody
      • MGC138257 antibody
      • MGC138285 antibody
      • mKIAA1150 antibody
      • p66/p68 antibody
      • P66B_HUMAN antibody
      • P66beta antibody
      • RGD1308533 antibody
      • transcription repressor p66 beta component of the MeCP1 complex antibody
      • Transcriptional repressor p66 beta antibody
      • Transcriptional repressor p66-beta antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling p66 beta with ab75280 at 1/200 (1µg/ml). Detection: DAB.
    • Detection of p66 beta by Western Blot of Immunprecipitate. anti-p66 beta at 1µg/ml staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab75280 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab75280 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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