Overview

  • Product name
  • Description
    Rabbit polyclonal to p66 beta
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human p66 beta (NP_065750.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab76924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 GATA-type zinc finger.
  • Domain
    CR1 is required for interaction with MBD3 and transcription repression.
    CR2 is required for localization to discrete loci in the nucleus.
  • Cellular localization
    Nucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37346 antibody
    • GATA zinc finger domain containing 2B antibody
    • GATA zinc finger domain containing protein 2B antibody
    • GATA zinc finger domain-containing protein 2B antibody
    • GATAD2B antibody
    • KIAA1150 antibody
    • MGC138257 antibody
    • MGC138285 antibody
    • mKIAA1150 antibody
    • p66/p68 antibody
    • P66B_HUMAN antibody
    • P66beta antibody
    • RGD1308533 antibody
    • transcription repressor p66 beta component of the MeCP1 complex antibody
    • Transcriptional repressor p66 beta antibody
    • Transcriptional repressor p66-beta antibody
    see all

Images

  • All lanes : Anti-p66 beta antibody (ab76924) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of p66 beta by Western Blot of Immunprecipitate.
    ab76924 at 1µg/ml staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab76924 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:
  • Chalei V  et al. The long non-coding RNA Dali is an epigenetic regulator of neural differentiation. Elife 3:e04530 (2014). Read more (PubMed: 25415054) »
  • Cui S  et al. Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic {beta}-Type Globin Promoters in Differentiated Adult Erythroid Cells. Mol Cell Biol 31:3298-311 (2011). Read more (PubMed: 21670149) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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