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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab76925 was affinity purified using an epitope specific to p66 beta immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
Use at 2-5 µg/mg of lysate.
Function Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
Tissue specificity Ubiquitous.
Sequence similarities Contains 1 GATA-type zinc finger.
Domain CR1 is required for interaction with MBD3 and transcription repression. CR2 is required for localization to discrete loci in the nucleus.
Cellular localization Nucleus. Nuclear, in discrete foci.
Information by UniProt
GATA zinc finger domain containing 2B antibody
GATA zinc finger domain containing protein 2B antibody
Anti-p66 beta antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p66 beta antibody (ab76925)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling p66 beta with ab76925 at 1/200 (1µg/ml). Detection: DAB.
Western blot - p66 beta antibody (ab76925)
All lanes : Anti-p66 beta antibody (ab76925) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size : 66 kDa Observed band size : 72 kDa ( why is the actual band size different from the predicted?) Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - p66 beta antibody (ab76925)
ab76925, at 1µg/ml, staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab76925 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
References for Anti-p66 beta antibody (ab76925)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"