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Rabbit polyclonal to p66 beta
Predicted to work with:
Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to a region between residues 50 and 100 of human p66 beta (NP_065750.1)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab76925 was affinity purified using an epitope specific to p66 beta immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
Use at 2-5 µg/mg of lysate.
Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
Contains 1 GATA-type zinc finger.
CR1 is required for interaction with MBD3 and transcription repression.
CR2 is required for localization to discrete loci in the nucleus.
Nucleus. Nuclear, in discrete foci.
Information by UniProt
GATA zinc finger domain containing 2B antibody
GATA zinc finger domain containing protein 2B antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p66 beta antibody (ab76925)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling p66 beta with ab76925 at 1/200 (1µg/ml). Detection: DAB.
Western blot - p66 beta antibody (ab76925)
All lanes :
Anti-p66 beta antibody (ab76925) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Predicted band size :
Observed band size :
72 kDa (
why is the actual band size different from the predicted?
Additional bands at :
130 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - p66 beta antibody (ab76925)
ab76925, at 1µg/ml, staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab76925 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"