Overview

  • Product nameAnti-p66 beta antibody
    See all p66 beta primary antibodies
  • Description
    Rabbit polyclonal to p66 beta
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to a region between residues 50 and 100 of human p66 beta (NP_065750.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionHas transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 GATA-type zinc finger.
  • DomainCR1 is required for interaction with MBD3 and transcription repression.
    CR2 is required for localization to discrete loci in the nucleus.
  • Cellular localizationNucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37346 antibody
    • GATA zinc finger domain containing 2B antibody
    • GATA zinc finger domain containing protein 2B antibody
    • GATA zinc finger domain-containing protein 2B antibody
    • GATAD2B antibody
    • KIAA1150 antibody
    • MGC138257 antibody
    • MGC138285 antibody
    • mKIAA1150 antibody
    • p66/p68 antibody
    • P66B_HUMAN antibody
    • P66beta antibody
    • RGD1308533 antibody
    • transcription repressor p66 beta component of the MeCP1 complex antibody
    • Transcriptional repressor p66 beta antibody
    • Transcriptional repressor p66-beta antibody
    see all

Anti-p66 beta antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling p66 beta with ab76925 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-p66 beta antibody (ab76925) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
  • ab76925, at 1µg/ml, staining p66 beta in HeLa whole cell lysate immunoprecipitated using ab76925 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

References for Anti-p66 beta antibody (ab76925)

ab76925 has not yet been referenced specifically in any publications.

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