Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208)

Overview

  • Product nameAnti-P70 S6 Kinase alpha (phospho T229) antibody
    See all P70 S6 Kinase alpha primary antibodies
  • Description
    Rabbit polyclonal to P70 S6 Kinase alpha (phospho T229)
  • SpecificityDetects endogenous levels of P70 S6 Kinase alpha only when phosphorylated at threonine 229 for human and rat, threonine 252 for mouse. This antibody was specifically designed for detection of S6K1 and binds to the protein kinase domain of isoforms alpha I and alpha II. We have some feedback to indicate that is may not be able to detect S6K2, but this has not been confirmed.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 229 (THTPFC)

  • Positive control
    • Human brain. Jurkat cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • RelevanceP70 S6 Kinase alpha is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and contains 2 non-identical kinase catalytic domains. It phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein is activated by serine/threonine phosphorylation and protein kinase C and inactivated by type 2A phosphatase. Activation leads to an increase in protein synthesis and cell proliferation. Overexpression of this kinase is involved in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
  • Cellular localizationCytoplasmic and synaptosome
  • Database links
  • Alternative names
    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • EC 2.7.11.1 antibody
    • KS6B1 antibody
    • p70 alpha antibody
    • p70 antibody
    • p70 S6 kinase alpha antibody
    • p70 S6K antibody
    • p70(S6K)-alpha antibody
    • p70S6K antibody
    • PS6K antibody
    • ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • Ribosomal protein S6, 70-KD, 1 antibody
    • RPS6KB1 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • serine/threonine kinase 14 alpha antibody
    • serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

Anti-P70 S6 Kinase alpha (phospho T229) antibody images

  • All lanes : Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunizing phosphopeptide


    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59208 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
  • Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution + Lysates prepared from human HT1080 cell line at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208) at 1/500 dilution + Lysate prepared from mouse NIH 3T3 cells at 10 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    This image is a courtesy of Anonymous Abreview

    See Abreview

References for Anti-P70 S6 Kinase alpha (phospho T229) antibody (ab59208)

ab59208 has not yet been referenced specifically in any publications.

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Thanks for your phone call today and for letting us know about the trouble with ab59208 in Western blot. As we discussed, I am sending a vial of ab48394 free of charge on the order ***. This product is in stock and should arrive tomorrow. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080 cell line)
Loading amount 10 µg
Specification HT1080 cell line
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Apr 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cell line)
Loading amount 10 µg
Specification NIH 3T3 cell line
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"